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ps6(s240/244) (cell signaling, cat# 5364  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ps6(s240/244) (cell signaling, cat# 5364
    Ps6(s240/244) (Cell Signaling, Cat# 5364, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ps6+s240/bio_rxiv__64898__2026__04__04__716461-206-119-120
    Average 86 stars, based on 1 article reviews
    ps6(s240/244) (cell signaling, cat# 5364 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Loss of O-GlcNAcylation modulates mTORC1 and autophagy in β cells, driving diabetes 2 progression
    Article Snippet: .. The sections were then incubated overnight at 4°C with primary antibodies against insulin (R00261-2; DAKO), RL2 (ab2739; Abcam), pS6 S240 (5364; Cell Signaling Technology), or LC3B (3868; Cell Signaling Technology). ..

    Article Title: O-GlcNAcylation modulates mTORC1 and autophagy in β-cells, driving diabetes progression.
    Article Snippet: .. The 450 sections were then incubated overnight at 4°C with primary antibodies against insulin (R00261-2; 451 DAKO), RL2 (ab2739; Abcam), pS6 S240 (5364; CST), or LC3B (3868; CST). ..

    Purification:

    Article Title: Overexpression of wild type RRAS2, without oncogenic mutations, drives chronic lymphocytic leukemia.
    Article Snippet: Fresh human PMBCs were obtained by density centrifugation in a LymphoprepTM (StemCell technologies) gradient of whole blood for flow cytometry and RT-qPCR analysis. .. Antibodies used were: anti-mouse IgM-, IgD-, CD21-, CD23-, B220-, CD45R-V450 –biotin –APC, CD11c(HL3), CD3- (145-2C11), CD4-, CD8a-, Gr1- (RB68C5) and NK1.1- (RA3-6B2) –biotin, CD5–PE (53–7.3), CD11b–PerCP-Cy5.5 (M1/70), purified CD16/32 (2,4G2), CD19-PE-Cy7 (1D3), CD45.1 -APC-Cy7 (A20), CD45.2 -APC (104), and AlexaFluor® 647 pBtk (Y223) from BD Pharmingen; anti-human RRAS2 from Abnova, anti-human β-Actin from Sigma-Aldrich (A228), antimouse F4/80 –biotin (BM8), from eBioscience; rabbit anti-mouse pERK (T202/Y204) (9101 L), pAKT (S473) (4060 L), pS6 (S240) (5364S), p4EBP1 (T37/46) (236B4), and pBLNK (Y96) from Cell Signalling; pVAV (Y174) (EP510Y) from Abcam, anti-IgM from Jackson Immunoresearch and anti-HA (12CA5) from Sigma. (See figure on next page.) ..

    Article Title: Overexpression of wild type RRAS2 , without oncogenic mutations, drives chronic lymphocytic leukemia
    Article Snippet: Fresh human PMBCs were obtained by density centrifugation in a LymphoprepTM (StemCell technologies) gradient of whole blood for flow cytometry and RT-qPCR analysis. .. Antibodies used were: anti-mouse IgM-, IgD-, CD21-, CD23-, B220-, CD45R-V450 –biotin –APC, CD11c- (HL3), CD3- (145-2C11), CD4-, CD8a-, Gr1- (RB6-8C5) and NK1.1- (RA3-6B2) –biotin, CD5–PE (53–7.3), CD11b–PerCP-Cy5.5 (M1/70), purified CD16/32 (2,4G2), CD19-PE-Cy7 (1D3), CD45.1 -APC-Cy7 (A20), CD45.2 -APC (104), and AlexaFluor® 647 pBtk (Y223) from BD Pharmingen; anti-human RRAS2 from Abnova, anti-human β-Actin from Sigma-Aldrich (A228), anti-mouse F4/80 –biotin (BM8), from eBioscience; rabbit anti-mouse pERK (T202/Y204) (9101 L), pAKT (S473) (4060 L), pS6 (S240) (5364S), p4EBP1 (T37/46) (236B4), and pBLNK (Y96) from Cell Signalling; pVAV (Y174) (EP510Y) from Abcam, anti-IgM from Jackson Immunoresearch and anti-HA (12CA5) from Sigma. ..

    BIA-KA:

    Article Title: Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
    Article Snippet: Isolated islets from 6 male and 6 female mice were sonicated in 1 × RIPA buffer (CST) with protease and phosphatase inhibitor cocktails (Abcam). .. Following BCA assay for protein quantification, lysates were resolved by SDS-PAGE, transferred to polyvinylidene fluoride membrane, and probed using primary antibody for RL2 (Abcam, AB_303264), O-GlcNAc transferase (Ogt; CST, AB_2716710), pS6 S240 (CST, AB_10694233), S6 (SCBT, AB_1129205), pAkt S473 (CST, AB_2315049), Akt (CST, AB_1147620), Kir6.2 (SCBT, AB_2721772), Pdx1 (Millipore, AB_10617514), and Glut2 (Millipore, AB_2890623). .. Using horseradish peroxidase-conjugated secondary antibody (GE), the blots were imaged with chemiluminescence.

    Membrane:

    Article Title: Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
    Article Snippet: Isolated islets from 6 male and 6 female mice were sonicated in 1 × RIPA buffer (CST) with protease and phosphatase inhibitor cocktails (Abcam). .. Following BCA assay for protein quantification, lysates were resolved by SDS-PAGE, transferred to polyvinylidene fluoride membrane, and probed using primary antibody for RL2 (Abcam, AB_303264), O-GlcNAc transferase (Ogt; CST, AB_2716710), pS6 S240 (CST, AB_10694233), S6 (SCBT, AB_1129205), pAkt S473 (CST, AB_2315049), Akt (CST, AB_1147620), Kir6.2 (SCBT, AB_2721772), Pdx1 (Millipore, AB_10617514), and Glut2 (Millipore, AB_2890623). .. Using horseradish peroxidase-conjugated secondary antibody (GE), the blots were imaged with chemiluminescence.



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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of <t>pS6</t> + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.
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    Image Search Results


    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of pS6 + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.

    Journal: EMBO Molecular Medicine

    Article Title: A viral glycoprotein targets IgG + memory B cells to mediate humoral immune evasion

    doi: 10.1038/s44321-026-00372-1

    Figure Lengend Snippet: ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of pS6 + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.

    Article Snippet: Phosphorylated proteins were detected using the following antibodies: pLyn (Tyr 397), pPDK1 (S241) (clone C49H2), pAKT (S473), pAKT (T308), pS6 (S240/244) (all Cell Signaling Technology), pPLCγ2 (pY759) (Miltenyi Biotec), and pSyk (Y348) (BD Biosciences).

    Techniques: Flow Cytometry, SDS Page, Control, Expressing

    ( A ) Phosphorylation of PDK1 and AKT at the indicated amino acid residues after stimulation of isolated B cells with 10 µg/mL of α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. Bars represent percentages of PDK1-positive IgG + B cells or MFI of pAKT (T308) and pAKT (S473) in IgG + B cells. At least three replicates were analyzed, and differences were analyzed using one-way ANOVA for PDK1 (**** P < 0.0001) or a Kruskal–Wallis test for pAKT (T308, * P = 0.0343) and pAKT (S473, * P = 0.023). Error bars + standard deviation of the mean. ( B ) B cells were incubated for 15 min with 2 µM Wortmannin or DMSO prior to stimulation with α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. pAKT (S473) was analyzed by flow cytometry ( n = 2). ( C ) Gating strategy to identify IgG + cells in the live B cells gate expressing phosphorylated S6. ( D ) Histograms show the MFI of p-S6 within the IgG + B cells gate. Bars represent the percentage of pS6-positive cells within the IgG + B cell population. Data represent five independent experiments; error bars indicate standard deviation, and differences between groups were analyzed using one-way ANOVA (** P = 0.0047).

    Journal: EMBO Molecular Medicine

    Article Title: A viral glycoprotein targets IgG + memory B cells to mediate humoral immune evasion

    doi: 10.1038/s44321-026-00372-1

    Figure Lengend Snippet: ( A ) Phosphorylation of PDK1 and AKT at the indicated amino acid residues after stimulation of isolated B cells with 10 µg/mL of α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. Bars represent percentages of PDK1-positive IgG + B cells or MFI of pAKT (T308) and pAKT (S473) in IgG + B cells. At least three replicates were analyzed, and differences were analyzed using one-way ANOVA for PDK1 (**** P < 0.0001) or a Kruskal–Wallis test for pAKT (T308, * P = 0.0343) and pAKT (S473, * P = 0.023). Error bars + standard deviation of the mean. ( B ) B cells were incubated for 15 min with 2 µM Wortmannin or DMSO prior to stimulation with α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. pAKT (S473) was analyzed by flow cytometry ( n = 2). ( C ) Gating strategy to identify IgG + cells in the live B cells gate expressing phosphorylated S6. ( D ) Histograms show the MFI of p-S6 within the IgG + B cells gate. Bars represent the percentage of pS6-positive cells within the IgG + B cell population. Data represent five independent experiments; error bars indicate standard deviation, and differences between groups were analyzed using one-way ANOVA (** P = 0.0047).

    Article Snippet: Phosphorylated proteins were detected using the following antibodies: pLyn (Tyr 397), pPDK1 (S241) (clone C49H2), pAKT (S473), pAKT (T308), pS6 (S240/244) (all Cell Signaling Technology), pPLCγ2 (pY759) (Miltenyi Biotec), and pSyk (Y348) (BD Biosciences).

    Techniques: Phospho-proteomics, Isolation, Standard Deviation, Incubation, Flow Cytometry, Expressing